pH-Mediated field-amplified sample stacking of pharmaceutical cations in high-ionic strength samples
نویسندگان
چکیده
منابع مشابه
Nonlinear Effects in Field Amplified Sample Stacking
In field amplified sample stacking (FASS), the sample solution is typically prepared in low conductivity buffer or DI water, so that sample ion concentration can be on the order of the buffer ion concentration. Under these conditions, sample ions can strongly affect conductivity gradients and this leads to nonlinear dispersive effects due to electromigration. We have developed a model to invest...
متن کاملDynamics of field-amplified sample stacking
Field-amplified sample stacking (FASS) uses conductivity gradients and resulting non-uniform electromigration fluxes to effect concentration increases of analyte ions. For cases where the initial sample concentration is much smaller than the background electrolyte (BGE) concentration, the ideal maximum concentration enhancement is equal to γ , the ratio of conductivity of the sample solution to...
متن کاملSingle-step Capillary Electrophoresis for Field-amplified Sample Stacking
We developed a novel single-step capillary electrophoresis (SSCE) scheme for field-amplified sample stacking (FASS) by using a hydrophilic material polymethyl methacrylate (PMMA) microchannel chip. The capillary effect was used to introduce liquids and control capillary stop valves in a partial barrier structure of the microchannel. Through the combined action of stop valves and air vents, both...
متن کاملEffect of a low-conductivity zone on field-amplified sample stacking in microchip micellar electrokinetic chromatography.
The effects of a low-conductivity (low-C) zone of an on-line sample preconcentration based on field-amplified sample stacking (FASS) under an acidic condition was investigated in microchip micellar electrokinetic chromatography (MCMEKC). By employing originally fabricated microchips with T- and cross-channel injectors on a single device, partial injection of the low-C zone and large-volume inj...
متن کاملOn-line sample preconcentration using field-amplified stacking injection in microchip capillary electrophoresis.
Previous reports describing sample stacking on microchip capillary electrophoresis (microCE) have regarded the microchip channels as a closed system and treated the bulk flow as in traditional capillary electrophoresis. This work demonstrates that the flows arising from the intersection should be investigated as an open system. It is shown that the pressure-driven flows into or from the branch ...
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ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2001
ISSN: 0173-0835,1522-2683
DOI: 10.1002/1522-2683(200101)22:1<59::aid-elps59>3.0.co;2-u